Journal: Türk Tarım ve Doğa Bilimleri Dergisi

Article Title: Ashwagandha root extract attenuates inflammation in Oleic acid induced-ALI/ARDS rat model via inhibition of ACE and MAPK signaling pathways

doi: 10.30910/turkjans.1209593

Figure Lengend Snippet: Figure 1. The effects of Ashwagandha on the MPO and MAPK levels on lung in ALI/ARDS. * denotes significant differences between other studied groups and control (*: p<0.05, ***: p<0.001), ε denotes significant differences between other studied groups and the OA group (ε: p<0.05, εε: p<0.01). Abbreviation used: OA: Oleic acid, Ash: Ashwagandha.

Article Snippet: The obtained supernatants were assayed for Myeloperoxidase (MPO), Glutathione (GSH), Superoxide Dismutase (SOD), Mitogen-activated protein kinases (MAPK), Angiotensin-converting enzyme (ACE) (BT LAB Company, China), and Total oxidant status (TOS) (Rel Assay Diagnostics, Gaziantep, Turkey) levels in rat lung tissue by enzyme-linked immunosorbent assay (ELISA) available kits in accordance with the manufacturer’s manuals.

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: qRT-PCR primers.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: Comparison of ERK5 expression in cardiomyocytes of various groups. Note: compared with the normal control group, ∗ P < 0.05; compared with the ADR group, # P < 0.05; compared with the ADR + LncRNA-MiR143HG silence + medicated serogroup, + P < 0.05.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Comparison, Expressing, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Wenyang Zhenshuai Granule on the Expression of LncRNA-MiR143HG/miR-143 Regulating ERK5 in H9C2 Cardiomyocytes

doi: 10.1155/2021/6431007

Figure Lengend Snippet: Comparison of the expression of p-ERK5 and ERK5 proteins in cardiomyocytes of each group. (a) Normal control group, (b) LncRNA-MiR143HG overexpression group, (c) LncRNA-MiR143HG silence group, (d) ADR group, (e) ADR + medicated serum group, (f) ADR + LncRNA-MiR143HG overexpression + medicated serogroup, and (g) ADR + LncRNA-MiR143HG silence + medicated serogroup. Compared with the normal control group, ∗ P < 0.05; compared with the ADR group, # P < 0.05; compared with the ADR + LncRNA-MiR143HG silence + medicated serogroup, + P < 0.05.

Article Snippet: Adriamycin hydrochloride was provided by Shenzhen Wanle Pharmaceutical Co., Ltd. (H44024359), SYBR Green PCR kit was purchased from Shanghai Sixin Biotechnology Co., Ltd. (BL705A), and Wenyang Zhenshuai granules were purchased from the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine Provided (201902), and ERK5 and p-ERK5 antibodies were purchased from Wuhan Boster Bioengineering Co., Ltd. (ab40908, ab5686).

Techniques: Comparison, Expressing, Control, Over Expression

Journal: Journal of Dental Sciences

Article Title: Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis

doi: 10.1016/j.jds.2020.07.013

Figure Lengend Snippet: Western blot analyses: phosphor-ERK and total-ERK in human oral cancer cell lines as compared with a primary culture of normal oral mucosa (HOK). Upregulation of phosphor-ERK (normalized to total-ERK) in OECM1 and Ca922 cell lines as compared with HOK, and a slightly decreased expression in SAS as compared with HOK. Results were quantified using densitometric analysis, normalized to the level of β-actin, and expressed as a fold change relative to the normal oral mucosa. Bars represent means ± standard deviation of the mean (∗ P < 0.05). A representative result of three independent experiments is shown.

Article Snippet: Further, samples were analyzed using 10% SDS-PAGE (Sigma-Aldrich) gels, and the proteins were transmitted onto a PVDF membrane (Sigma-Aldrich) using Bio-Rad's transblot with primary antibodies against phosphor-ERK (Boster Biological Technology, CA, USA; Cat. No. P00104; 1:1000) and total-ERK (Boster Biological Technology; Cat. No. P00104; 1:1000), with species specificity for human tissues and an observed molecular weight of 42–44 kDa; and β-actin (Sigma-Aldrich; 1:1000), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (Sigma-Aldrich; 1:5000).

Techniques: Western Blot, Expressing, Standard Deviation